Fig. 5
Schematic representation of CeDGAT2-2 gene expression cassette in the vectors. a Empty vector without the target gene sequence was used as vector control (pYES2.0); b CeDGAT2-2 expression cassette was inserted into a yeast vector pYES2.0; c empty vector without the target gene sequence was used as vector control (pBI121); d CeDGAT2-2 expression cassette was inserted into a plant vector pBI121. Amp, ampicillin resistance gene; URA3, selection gene of yeast transformants in uracil-deficient medium; 2μ ori, maintenance and high copy replication gene in yeast; f1 ori, rescue gene of single-stranded DNA; PGAL1, promoter; CYC1, transcription termination; pUC ori, maintenance and high copy replication gene in E. coli; Nos P, promoter; NTP II, neomycin phosphotransferase II gene; Nos T, termination; CaMV35S2, promoter; GUS, report gene. e Gel images showing double-digested fragments of the vectors. Lane 1‚ pBI121 recombinant plasmid with XbaI-KpnI; lane 2‚ pBI121 recombinant plasmid digested with XbaI; lane 3‚ pYES2.0 recombinant plasmid with XbaI-XmaI; lane 4‚ pYES2.0 recombinant plasmid digested with XbaI; lane M‚ 2000-bp DNA marker