Fig. 9

Total oil content (a) and fatty acid profiles (b) in N. tabacum leaves expressing CeDGAT2-2 and AtDGAT1 genes, respectively. WT, wild-type tobacco plant; EV, the tobacco plant expressing empty vector; AtDGAT1, the tobacco plant expressing AtDGAT1 gene; CeDGAT2-2, the tobacco plant expressing CeDGAT2-2 gene. Total oil content was expressed as percentage of leaf dry weight. The top fully expanded leaf from tobacco plants at floral transition stage was sampled. Lipids were extracted from the leaf samples and oil content was determined by the method described in the section of “Materials and methods”. For fatty acid profiling, lipids were transesterified, followed by gas chromatography (GC) analysis. Each value is the mean ± SE of six biological replicates. * and ** indicate statistically significant difference compared with the control (t-test) at P < 0.05 and P < 0.01, respectively