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Table 7 LC–MS identification of the main ions detected during oxidation of sinapic acid by PcLac1 and PcLac2

From: Discovery of two novel laccase-like multicopper oxidases from Pleurotus citrinopileatus and their application in phenolic oligomer synthesis

Compound Rt (min) m/z [M-H] MF MS/MS Identification PcLac1 samples PcLac2 samples
Dimers
17 6.19 445 C22H21O10 189 (C11H9O3), 174 (C10H6O3), 161 (C9H5O3), 145 (C9H5O2), 121 (C7H5O2) Disinapic acid Minor Major
18 6.69 445 C22H21O10 189 (C11H9O3), 174 (C10H6O3), 161 (C9H5O3), 145 (C9H5O2), 121 (C7H5O2) Disinapic acid Major Minor
19 6.97 401.1220 C21H21O8 189 (C11H9O3), 174 (C10H6O3), 161 (C9H5O3), 145 (C9H5O2),
133 (C8H5O2), 121 (C7H5O2)
Decarboxylated disinapic acid Not detected Detected
20 7.21 401.1220 C21H21O8 189 (C11H9O3), 174 (C10H6O3), 161 (C9H5O3), 145 (C9H5O2), 133 (C8H5O2), 121 (C7H5O2) Decarboxylated disinapic acid Not detected Detected
21 6.26 357.060 C18H13O8 271 (C14H7O6), 227 (C13H7O4), 199 (C12H7O2), 167 (C12H7O) Bis-Decarboxylated disinapic acid Detected Detected
Trimers
22 7.38 667.1643 C33H31O15 - Trisinapic acid Not detected Minor
23 7.09 623.1746 C32H31O13 265 (C16H9O4), 209 (C14H9O2), 121 (C7H5O2) Trisinapic acid, decarboxylated Not detected Detected
24 7.55 623.1746 C32H31O13 265 (C16H9O4), 209 (C14H9O2), 121 (C7H5O2) Trisinapic acid, decarboxylated Not detected Detected
25 8.16 579.1846 C31H31O11 339 (C19H15O6) Trisinapic acid, bis-decarboxylated Not detected Detected
Tetramers
26 6.98 891.2319 C44H43O20 341 (C19H17O6), 189 (C11H9O3), 174 (C10H6O3), 161 (C9H5O3), 145 (C9H5O2), 121 (C7H5O2) Tetrasinapic acid Not detected Detected
Others
27 7.99 503.1172 C24H23O12 147 (C8H3O3) 119 (C7H3O2) Unknown compound Detected Not detected