Fig. 5

Saccharification of hydrothermally pretreated sugarcane straw and pine. a Glucan and b xylan enzymatic conversion. The reaction was performed with a mixture of Celluclast®: glucosidase from Aspergillus niger (5:1 w/w) supplemented with L. sulphureus ATCC 52600 secretome produced on SCB. Total protein load corresponds to 15 FPU/g of substrate (100%). Reactions containing 2% (w/v) solids load in 50 mM sodium acetate pH 5.0 were incubated for 48 h at 50 °C. Data correspond to mean values and standard deviations of four triplicates. Significance was analyzed using two-way ANOVA with Tukey’s test relative to the reference reaction "85% Celluclast" (95% confidence interval) and is indicated as follows: *p < 0.05, **p < 0.01, ***p < 0.001