Table 1 Effect of CRISPR interference of the selected ATP-consuming enzyme-encoding genes on 4HPAA production in E. coli 4HPAA-2
Gene | Description | Ratio* |
|---|---|---|
purC | Phosphoribosylaminoimidazole-succinocarboxamide synthase. It catalyzes the reaction: ATP + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate + L-aspartate → ADP + 5′-phosphoribosyl-4-(N-succinocarboxamide)-5-aminoimidazole + phosphate + H+ | 1.38 ± 0.02 |
araH | Arabinose ABC transporter membrane subunit | 1.36 ± 0.04 |
yeaG | Protein kinase | 1.35 ± 0.03 |
sucC | Succinyl-CoA synthetase subunit β. It catalyzes the reaction: succinate + ATP + coenzyme A ↔ succinyl-CoA + ADP + phosphate | 1.34 ± 0.01 |
dppD | Dipeptide ABC transporter ATP-binding subunit | 1.33 ± 0.02 |
artP | L-Arginine ABC transporter ATP-binding subunit | 1.33 ± 0.02 |
fecE | ferric citrate ABC transporter ATP-binding subunit | 1.33 ± 0.03 |
artM | L-Arginine ABC transporter membrane subunit | 1.30 ± 0.02 |
argB | Acetylglutamate kinase. It catalyzes the reaction: N-acetyl-L-glutamate + ATP → N-acetylglutamyl-phosphate + ADP | 1.30 ± 0.02 |
mgtA | Mg2+ importing P-type ATPase | 1.24 ± 0.02 |
aas | Acyltransferase. It has long-chain fatty acid-CoA/long-chain fatty acid [acyl-carrier-protein] ligase activity | 1.22 ± 0.03 |
sapF | Putrescine ABC exporter ATP-binding protein | 1.21 ± 0.02 |
nanK | N-Acetylmannosamine kinase. It catalyzes the reaction: N-acetyl-D-mannosamine + ATP → N-acetyl-D-mannosamine 6-phosphate + ADP + H+ | 1.20 ± 0.03 |
phnN | Ribose 1,5-bisphosphate phosphokinase, It catalyzes the reaction: α-D-ribose 1,5-bisphosphate + ATP ↔ 5-phospho-α-D-ribose 1-diphosphate + ADP | 1.18 ± 0.02 |
pfkA | 6-Phosphofructokinase 1. It catalyzes the reaction: β-D-fructofuranose 6-phosphate + ATP → ADP + β-D-fructose 1,6-bisphosphate + H+ | 1.18 ± 0.02 |
ssuC | Aliphatic sulfonates transport permease protein | 1.17 ± 0.02 |
atpG | ATP synthase F1 complex subunit γ | 1.15 ± 0.01 |
copA | Soluble Cu+ chaperone | 1.11 ± 0.01 |
hisP | Histidine/lysine/arginine/ornithine transport ATP-binding protein | 1.09 ± 0.03 |