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Table 1 Effect of CRISPR interference of the selected ATP-consuming enzyme-encoding genes on 4HPAA production in E. coli 4HPAA-2

From: ATP and NADPH engineering of Escherichia coli to improve the production of 4-hydroxyphenylacetic acid using CRISPRi

Gene Description Ratio*
purC Phosphoribosylaminoimidazole-succinocarboxamide synthase. It catalyzes the reaction: ATP + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate + L-aspartate → ADP + 5′-phosphoribosyl-4-(N-succinocarboxamide)-5-aminoimidazole + phosphate + H+ 1.38 ± 0.02
araH Arabinose ABC transporter membrane subunit 1.36 ± 0.04
yeaG Protein kinase 1.35 ± 0.03
sucC Succinyl-CoA synthetase subunit β. It catalyzes the reaction: succinate + ATP + coenzyme A ↔ succinyl-CoA + ADP + phosphate 1.34 ± 0.01
dppD Dipeptide ABC transporter ATP-binding subunit 1.33 ± 0.02
artP L-Arginine ABC transporter ATP-binding subunit 1.33 ± 0.02
fecE ferric citrate ABC transporter ATP-binding subunit 1.33 ± 0.03
artM L-Arginine ABC transporter membrane subunit 1.30 ± 0.02
argB Acetylglutamate kinase. It catalyzes the reaction: N-acetyl-L-glutamate + ATP → N-acetylglutamyl-phosphate + ADP 1.30 ± 0.02
mgtA Mg2+ importing P-type ATPase 1.24 ± 0.02
aas Acyltransferase. It has long-chain fatty acid-CoA/long-chain fatty acid [acyl-carrier-protein] ligase activity 1.22 ± 0.03
sapF Putrescine ABC exporter ATP-binding protein 1.21 ± 0.02
nanK N-Acetylmannosamine kinase. It catalyzes the reaction: N-acetyl-D-mannosamine + ATP → N-acetyl-D-mannosamine 6-phosphate + ADP + H+ 1.20 ± 0.03
phnN Ribose 1,5-bisphosphate phosphokinase, It catalyzes the reaction: α-D-ribose 1,5-bisphosphate + ATP ↔ 5-phospho-α-D-ribose 1-diphosphate + ADP 1.18 ± 0.02
pfkA 6-Phosphofructokinase 1. It catalyzes the reaction: β-D-fructofuranose 6-phosphate + ATP → ADP + β-D-fructose 1,6-bisphosphate + H+ 1.18 ± 0.02
ssuC Aliphatic sulfonates transport permease protein 1.17 ± 0.02
atpG ATP synthase F1 complex subunit γ 1.15 ± 0.01
copA Soluble Cu+ chaperone 1.11 ± 0.01
hisP Histidine/lysine/arginine/ornithine transport ATP-binding protein 1.09 ± 0.03
  1. The data represent the means of three replicates, and error bars represent standard deviations