Fig. 8

The effect of T. reesei enzyme mixtures on enzymatic hydrolysis performance. Depicted are conversion yields for enzymatic hydrolysis of NBSK (a), LP-STEX (b), and LP-ALKOX (c) with Celluclast (CC) and Celluclast supplemented with BSA (CC + BSA), endo-mannanase (CC + MAN), and concentrated T. reesei QM6a cultivation supernatants (CC + P_NBSK, CC + P_LP-STEX, and CC + P_LP-ALKOX). All reactions were run at a CC protein loading of 20 mg g⁻¹ substrate dry mass. Supplementation of fungal protein, BSA, and mannanase was at a protein loading of 5 mg g⁻¹ substrate dry mass, as detailed in Methods section. As benchmark, hydrolysis reactions with Cellic Cetc3 at 10 (CT10) and 20 (CT20) FPU g⁻¹ dry mass are also depicted. All data represent mean values from duplicate experiments and error bars represent the spread