Fig. 2

Construction of OmcS introduced and d-lactate-producing strains. a Genetic modifications and d-lactate production in all strains. b Genome PCR confirmed the integration of omcS and Idh genes into the genome of all mutants. c RT-PCR confirmation of expression of omcS and ldh in corresponding strains. Rubisco large subunit rbcL was used as positive control. d Comparable analysis of omcS and ldh gene expression in corresponding strains at 12 and 36 h during rapid d-lactate production period by transcriptome. e Ldh expression in corresponding strains by 12% SDS-PAGE analysis. Expression level of Ldh under control of strong promoter P_cpc560 was approximately 10% of total soluble protein, whereas OmcS (47 kDa) expressed under control of P_rbcL200 was almost undetectable on the gel