Skip to main content

Table 1 Strains and plasmids used in this study

From: Engineering oleaginous yeast Rhodotorula toruloides for overproduction of fatty acid ethyl esters

Strains or plasmids

Relevant characteristics

Source or reference

Strains

 Δku70

R. toruloides IFO0880 deficient in ku70

[34]

 Δku70-AbWS

Δku70 strain harboring the AbWS cassette

This study

 Δku70-AbWS*

Δku70 strain harboring the AbWS* cassette

This study

 Δku70-MmWS

Δku70 strain harboring the MmWS cassette

This study

 Δku70-MhWS

Δku70 strain harboring the MhWS cassette

This study

 Δku70-PaWS

Δku70 strain harboring the PaWS cassette

This study

 Δku70-RoWS

Δku70 strain harboring the RoWS cassette

This study

 AGL-1

A. tumefaciens strain AGL0 recA::bla pTiBo542ΔT Mop+ CbR

[15]

 DH5α

E. coli (supE44 lacU169 hsdR17 recA1 endA1 gyrA96 thi-l relA1)

Laboratory storage

Plasmids

 pKOCAR2

Harboring the hygromycin selection cassette for target gene incorporation at the specific site in CAR2 gene

[32]

 p101

Harboring the MhWS cassette

This study

 p102

Harboring the AbWS cassette

This study

 p103

Harboring the RoWS cassette

This study

 p104

Harboring the MmWS cassette

This study

 p105

Harboring the PaWS cassette

This study

 p1022

Harboring the AbWS* cassette

This study