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Table 1 Comparison of growth characteristics and products of E. limosum NG-6894 cultivated using various carbon and energy sources

From: Production of the biocommodities butanol and acetone from methanol with fluorescent FAST-tagged proteins using metabolically engineered strains of Eubacterium limosum

Substrate Max. OD600 Growth rate (h−1) Doubling time (h) Products (mM) Products (mol/100 mol substrate) Product ratio (acetate:butyrate)a
Acetate Butyrate Acetate Butyrate
Glucose
30 mM 2.8 0.23 3 55 9.7 183.2 32.4 6:1
Methanol
50 mM 1.4 0.09 7.7 15.8 8 29.5 14.8 2:1
100 mM 2 0.09 7.7 28.4 13.8 26.6 12.9 2:1
200 mM 2.6 0.09 7.7 42.8 24.7 22.8 13.1 2:1
Gases
H2 + CO2 0.6 0.02 34.7 85 2.4 n.d. n.d. 36:1
Syngas 2.9 0.06 11.6 65.5 14.5 n.d. n.d. 9:2
  1. aProduct ratios were calculated for products in (mM) and (mol/100 mol substrate)
  2. n.d. not determined