Production of the biocommodities butanol and acetone from methanol with fluorescent FAST-tagged proteins using metabolically engineered strains of Eubacterium limosum

Table 1 Comparison of growth characteristics and products of E. limosum NG-6894 cultivated using various carbon and energy sources

Substrate	Max. OD₆₀₀	Growth rate (h⁻¹)	Doubling time (h)	Products (mM)		Products (mol/100 mol substrate)		Product ratio (acetate:butyrate)^a
Substrate	Max. OD₆₀₀	Growth rate (h⁻¹)	Doubling time (h)	Acetate	Butyrate	Acetate	Butyrate	Product ratio (acetate:butyrate)^a
Glucose
30 mM	2.8	0.23	3	55	9.7	183.2	32.4	6:1
Methanol
50 mM	1.4	0.09	7.7	15.8	8	29.5	14.8	2:1
100 mM	2	0.09	7.7	28.4	13.8	26.6	12.9	2:1
200 mM	2.6	0.09	7.7	42.8	24.7	22.8	13.1	2:1
Gases
H₂ + CO₂	0.6	0.02	34.7	85	2.4	n.d.	n.d.	36:1
Syngas	2.9	0.06	11.6	65.5	14.5	n.d.	n.d.	9:2

^aProduct ratios were calculated for products in (mM) and (mol/100 mol substrate)
n.d. not determined

ISSN: 2731-3654