Production of the biocommodities butanol and acetone from methanol with fluorescent FAST-tagged proteins using metabolically engineered strains of Eubacterium limosum

Table 2 Growth characteristics, ethanol, and butanol production of recombinant E. limosum strains characterized in growth experiments using glucose as carbon source

Recombinant E. limosum strains	OD_max	Growth rate (h⁻¹)	Ethanol (mM)	Butanol (mM)
pMTL83251_P_bgaL_AdhE2
Induced	2.5	0.17	1.9	1.6
Non-induced	2.8	0.2	0.3	0.3
pMTL83251_P_bgaL_C-FAST-AdhE2
Induced	2.5	0.16	1	0.6
Non-induced	3	0.17	0.3	0
pMTL83251_P_bgaL_N-FAST-AdhE2
Induced	2.7	0.18	2.4	1.8
Non-induced	3	0.22	0.4	0.2

ISSN: 2731-3654