Table 1 The kinetic parameters of the three recombinant xylanases
From: Synergistic mechanism of GH11 xylanases with different action modes from Aspergillus niger An76
Enzyme | Substrate | Specific activity (IU/mg) | Vmax (μmol/min/mg) | Km (mg/mL) | kcat (s−1) | kcat/Km (mL/mg/s) |
|---|---|---|---|---|---|---|
XynA | Beechwood xylan | 139.9 | 1446.0 | 10.7 | 481.8 | 45.2 |
XynB | Beechwood xylan | 1146.3 | 9330.0 | 3.0 | 3460.3 | 1140.9 |
XynD | Beechwood xylan | 170.5 | 2813.0 | 5.4 | 1080.2 | 199.5 |
XynA | Wheat arabinoxylan | 99.6 | 904.3 | 10.2 | 301.3 | 29.5 |
XynB | Wheat arabinoxylan | 1019.3 | 5172.0 | 3.7 | 1918.2 | 512.3 |
XynD | Wheat arabinoxylan | 160.8 | 1319.0 | 6.3 | 506.4 | 79.9 |