Novel molecular biological tools for the efficient expression of fungal lytic polysaccharide monooxygenases in Pichia pastoris

Table 5 List with the theoretical peptide masses of the trypsin digested NcAA9C expressed with the different signal peptides

Fragment	Masses expected if the signal peptide is cleaved correctly	Masses expected of a wrongly processed N-terminus
Fragment	Independent of SP	Native	OST1	pre- OST1-pro-α-factor
1	773.4304	1986.1018	3104.5855	6754.3264
2	1215.6328	1215.6328	1215.6328	890.4465
3	2505.1782	2505.1782	2505.1782	1173.5898
4	1171.6317	1171.6317	1171.6317	1215.6328
5	2725.2865	2725.2865	2725.2865	2505.1782
6	991.5645	991.5645	991.5645	1171.6317
7	1234.6273	1234.6273	1234.6273	2725.2865
8	945.4999	945.4999	945.4999	991.5645
9	3347.5322	3347.5322	3347.5322	1234.6273
10	5926.898	5926.898	5926.898	945.4999
11	6678.2651	6678.2651	6678.2651	3347.5322
12	1020.5142	1020.5142	1020.5142	5926.898
13	1469.7091	1469.7091	1469.7091	6678.2651
14	1038.5037	1038.5037	1038.5037	1020.5142
15	1355.5718	1355.5718	1355.5718	1469.7091
16	1359.5674	1359.5674	1359.5674	1038.5037
17				1355.5718
18				1359.5674

The second column shows the expected masses of a correctly processed enzyme and is therefore independent of the leading sequence. In the columns 3 to 5 the expected masses of wrongly processed N-termini of the different signal peptides are listed. Highlighted in bold are the masses of the peptides containing the N-terminal histidine

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