Fig. 1

S. cerevisiae-expressed D2-BGL mutants show higher specific activity than the wild-type D2-BGL (WT D2). a, b Specific activity was determined at 10 mM cellobiose (a) and 50 mM cellobiose (b) with Mut A (D358G + S496P, in red), Mut B (D224G + S592T, in blue) and Mut C (F256M + Y260D, in green). c Locations of the six amino acid replacements on the three-dimensional protein structure of D2-BGL (PDB: 6JXG). Experiments were performed in triplicate, and error bars represent the standard deviation. *: P value < 0.05 (t test)