Fig. 3

Mut M expressed in P. pastoris shows higher cellobiase activity than that of WT. a Growth curves of P. pastoris strains expressing WT D2 and Mut M. b Cellobiase activity measured with culture supernatant from WT D2 and Mut M at 10 mM cellobiose. c Stability assay with samples incubated at different pH with preservation at 4 °C for 24 h. d Stability assay with samples incubated at different temperatures for 4 h. Experiments were performed at least in triplicate, and error bars represent the standard deviation