Skip to main content

Table 2 Kinetic parameters of WT D2, F256M and Mut M enzymes

From: Improvements of the productivity and saccharification efficiency of the cellulolytic β-glucosidase D2-BGL in Pichia pastoris via directed evolution

  WT D2 F256M Mut M
Substrate
 Cellobiose
  Vmax (U/L) 371 ± 15 584 ± 13 469 ± 13
  Km (mM) 1.28 ± 0.20 1.51 ± 0.13 1.69 ± 0.17
  Ki substrate (mM) 133 ± 25 208 ± 28 200 ± 32
 pNPG
  Vmax (U/L) 575 ± 110 ND 374 ± 30
  Km (mM) 0.31 ± 0.14 ND 0.18 ± 0.05
  Ki substrate (mM) 2.19 ± 0.75 ND 3.21 ± 0.56
  Ki glucose (mM) 3.23 ± 1.08 ND 1.59 ± 0.07
  1. ND not determined