Table 2 Kinetic parameters of WT D2, F256M and Mut M enzymes
WT D2 | F256M | Mut M | |
|---|---|---|---|
Substrate | |||
Cellobiose | |||
Vmax (U/L) | 371 ± 15 | 584 ± 13 | 469 ± 13 |
Km (mM) | 1.28 ± 0.20 | 1.51 ± 0.13 | 1.69 ± 0.17 |
Ki substrate (mM) | 133 ± 25 | 208 ± 28 | 200 ± 32 |
pNPG | |||
Vmax (U/L) | 575 ± 110 | ND | 374 ± 30 |
Km (mM) | 0.31 ± 0.14 | ND | 0.18 ± 0.05 |
Ki substrate (mM) | 2.19 ± 0.75 | ND | 3.21 ± 0.56 |
Ki glucose (mM) | 3.23 ± 1.08 | ND | 1.59 ± 0.07 |