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Fig. 4 | Biotechnology for Biofuels

Fig. 4

From: Polysaccharide utilization loci-driven enzyme discovery reveals BD-FAE: a bifunctional feruloyl and acetyl xylan esterase active on complex natural xylans

Fig. 4

Comparison of the 1H-NMR spectra of X2Ac5 and X2Ac4 substrate blanks in CDCl3 and the product of X2Ac5 after treatment with BD-FAE extracted into CDCl3 showing the disappearance of the acetyl signal corresponding to 1-O-Ac and formation of a signal pattern corresponding to X2Ac4 (where the signal of 2-O-Ac shifts to overlap with the other acetyl signals)

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