Kinetic modeling of phosphorylase-catalyzed iterative β-1,4-glycosylation for degree of polymerization-controlled synthesis of soluble cello-oligosaccharides

Table 3 Collection of R² values obtained from fitting time courses generated for experiments A–D with models PE18, PE19 and PE20

Model	Experiment αGlc1-P/G2	Cellobiose	G3	G4	G5	G6	Phosphate
PE18	0.2	0.904	0.908	0.869			0.966
PE19		0.904	0.908	0.868			0.966
PE20		0.904	0.908	0.866			0.966
PE18	1.0	0.993	0.920	0.965	0.919	0.942	0.973
PE19		0.994	0.919	0.965	0.919	0.942	0.973
PE20		0.992	0.921	0.966	0.921	0.941	0.972
PE18	2.0	0.905	0.937	0.987	0.881	0.686	0.976
PE19		0.905	0.937	0.987	0.882	0.685	0.976
PE20		0.905	0.937	0.988	0.886	0.679	0.977
PE18	4.0	0.967	0.960	0.990	0.969	0.756	0.993
PE19		0.967	0.961	0.990	0.969	0.754	0.993
PE20		0.967	0.960	0.990	0.970	0.757	0.993

ISSN: 2731-3654