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Table 1 Substrate specificity of FgrAAO and FoxAAO

From: Two Fusarium copper radical oxidases with high activity on aryl alcohols

Substrate FgrAAO FoxAAO
KM (mM) kcat (s−1) kcat/KM (M−1 s−1) KM (mM) kcat (s−1) kcat/KM (M−1 s−1)
Carbohydrates
 Galactose 1700 ± 150 21 ± 1.0 12 1600 ± 150 23 ± 1.2 14
 Galactose 450 ± 74 36 ± 1.2 79 620 ± 57 19 ± 0.35 31
 Fructose 460 ± 91 9.7 ± 0.55 21 510 ± 82 16 ± 0.72 31
Polyols
 Glycerol 2200 ± 360 17 ± 1.2 7.7 2700 ± 403 24 ± 1.6 8.8
Aryl alcohols
 Benzyl alcohol 86 ± 12 37 ± 2.8 430 30 ± 19 31 ± 4.2 1000 (350 ± 20)a
 Cinnamyl alcohol n.d.b n.d.b 3000 n.d.b n.d.b 3200
 4-Methoxybenzyl (p-anisyl) alcohol 59 ± 7.2 32 ± 1.8 540 51 ± 12 34 ± 2.9 290
 3-Methoxybenzyl (m-anisyl) alcohol 6.5 ± 1.5 52 ± 1.5 8000 6.7 ± 0.59 34 ± 1.7 5100
 3,4-Dimethoxybenzyl (veratryl) alcohol 3.7 ± 0.75 41 ± 4.0 11000 3.0 ± 0.24 25 ± 1.2 8300
Furans
 HMF 14 ± 2.6 29 ± 1.7 2100 17 ± 4.9 26 ± 2.6 1500
 DFF 10 ± 2.9 3.6 ± 0.27 360 3.7 ± 1.0 1.4 ± 0.13 380
 HMFCA 4.4 ± 0.90 3.8 ± 0.33 860 3.1 ± 0.31 5.2 ± 0.14 1700
  1. Errors represent the standard deviation from the mean values
  2. aKM, kcat, and kcat/KM values calculated from fitting of the standard Michaelis–Menten equation to the data. Value in parentheses is the kcat/KM value obtained from a linear fit to the data in the substrate range 1–50 mM. Fitting of the data with a modified Michaelis–Menten equation accounting for substrate inhibition was unsuccessful due to very high errors on individual parameters (see Additional file 1: Figure S6)
  3. bIndividual KM and kcat values not determinable due to substrate solubility limitations; kcat/KM values obtained from slope of linear v0 versus [S] plots (Additional file 1: Figure S6)