Fig. 2
From: Modular biosynthesis of plant hemicellulose and its impact on yeast cells
Abundance and composition of engineered wall polymers. a Relative green fluorescence of independent Pichia colonies (except that sfGFP controls are biological replicates). b Mannose (Man) content as a percent of the total sugars detected for the alkaline-insoluble polymers (made by the cells from a). c, d Absolute monosaccharide composition of alkaline-insoluble polymers after cultivation of engineered yeast strains in two media: c YPM (containing 1.5% w/w Methanol as sole carbon source) or d YPM + G (plus 0.5% v/v Glycerol to boost biomass accumulation. Polysaccharides were hydrolyzed using sulfuric acid. In c, d dots show the values of two biological replicates, and significant differences between samples are marked by different letters (one-way ANOVA with Tukey test, P < 0.05). All samples in d had similar Glc levels