Fig. 5

CSLA expression influences yeast growth and cell wall morphology. a Growth curve of CSLA parents and top swaps after transfer to YPM in a 48-well plate. Optical density at 600 nm (OD600) and yellow relative fluorescence units (RFU) were monitored with a plate reader after every 30 min of shaking. Data show the mean ± SD of at least three biological replicates. The error bars are only shown for Venus and AkCSLA3 due to space constraints, but data points had a coefficient of variance below 5% after 32 h. b Morphology of yeast cells stained with Trypan Blue (TB) after 72 h of cultivation in YPM + G. c Yeast cell area (µm²) of CSLA parents and top swaps. Violin plot shows the size distribution of at least 350 cells per genotype, and significant differences between samples are marked by different letters (one-way ANOVA with Tukey test, P < 0.0001). d Zymolyase and β-mercaptoethanol treatment spheroplasted Venus cells based on Transmitted Light (TL) and yellow protein fluorescence (YFP) imaging. Scale bars = 5 µm in b, 10 µm in d. Arrows indicate TB uptake