Fig. 4
ESI–MS of side chain B released from RG-II by selective acid hydrolysis is degraded during methylation with solid NaOH and methyl iodide in DMSO. a Methylated reducing B side chain oligosaccharides generated from W. repanda RG-II (left panel). The ms2 spectrum (middle panel) and fragmentation pattern of the major ion (m/z 1143; right panel) is also shown. b NaBH4-reduced and methylated B side chain oligosaccharides generated from W. repanda RG-II (left panel). The ms2 spectrum (middle panel) and fragmentation pattern of the major ion (m/z 1319; right panel) is also shown. c Methylated reducing B side chain oligosaccharides generated from celery RG-II (left panel). The ms2 spectrum (middle panel) and fragmentation pattern of the major ion (m/z 969; right panel) is also shown. d NaBH4-reduced and methylated B side chain oligosaccharides generated from celery RG-II (left panel). The ms2 spectrum (middle panel) and fragmentation pattern of the major ion (m/z 1145; right panel) is also shown. Refer to Fig. 1c for the relevant symbol nomenclature for glycans