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Fig. 4 | Biotechnology for Biofuels

Fig. 4

From: Engineering of Yarrowia lipolytica transporters for high-efficient production of biobased succinic acid from glucose

Fig. 4

Construction of the Y. lipolytica chassis cell through optimizing SA biosynthetic pathways. a Effect of the different genetic modifications on SA production of Y. lipolytica. To enhance the metabolic flux of reductive TCA, oxidative TCA and glyoxylate pathway, the expression cassettes of TbFrd, YlScs2 and YlYhm2-YlMls-YlIcl were overexpressed in PGC62 strain, respectively; b fermentation profile of the engineered strain PGC62-SYF with optimized SA biosynthetic pathway in shaking flasks. Error bars show the SDs of 3 biological replicates

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