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Fig. 7 | Biotechnology for Biofuels

Fig. 7

From: Investigation of the impact of a broad range of temperatures on the physiological and transcriptional profiles of Zymomonas mobilis ZM4 for high-temperature-tolerant recombinant strain development

Fig. 7

Schematic diagram of plasmids and promoter replacement method. The constructed pEZ_EGFP (a), long fragment for replacing the native promoter of target gene on the genome (b), and pEZ_Target (Target means one of these three constructs: clpB, an operon containing clpB_groESL with groESL driven by their native promoter, and ZMO0994) (c). PlacUV5 is a constitutive promoter. rrnB T1 and T7Te are terminators. Zymo Ori means an origin of replication with promoters for Z. mobilis. p15A Ori means an origin of replication with promoters for E. coli. P refers to the original promoter of the gene of interest. US means the upstream of the original promoter. aadA refers to spectinomycin resistance gene. Ptet is a bi-directional promoter, which simultaneously initiates the expression of tetR gene and the candidate gene. Ptet fragment included aadA, tetR, and Ptet

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Biotechnology for Biofuels and Bioproducts

ISSN: 2731-3654

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