Fig. 5

Production of isoamyl alcohol in engineered Y. lipolytica. The cultures were grown in 40 mL YPD medium with an initial OD₆₀₀ of 0.1 and 10% of n-dodecane in a 250 mL shake flask at 225 rpm and 28 °C for 3 days. The organic phase was analyzed by GC/MS. ScARO10 is the key gene in the heterologous isoamyl alcohol production pathway. The isoamyl alcohol titer of Po1g ScARO10, which expressed ScARO10 under P_UAS1B4-LEUm, was 1.36 mg/L, which was 2.7-fold higher than the control strain Po1g KU70Δ. The strain Po1g BAA, which co-expressed ScBAT1, ScARO10 and ScADH2 under P_UAS1B4-LEUm, achieved an isoamyl alcohol titer of 1.8 mg/L, which was 3.9-fold that of control strain Po1g KU70Δ. The error bars represent standard deviation