Fig. 2

ODH substrate screening. Histograms correspond to the amount of DCIP which is reduced over time using a set of 14 sugars as electron donors for ODH reaction. Columns are colored using a grey scale code from black to light grey (t = 2 min to t = 19 h, respectively). Sugar substrates were all tested at the same concentration (2.5 mM). They include the monosaccharides D-glucose (GLC), D-xylose (XYL), D-galactose (GAL) and D-mannose (MAN), the disaccharides D-lactose (LAC), D-maltose (MAL), cellobiose (G4G) and laminaribiose (G3G), the trisaccharides cellotriose (G4G4G), laminaritriose (G3G3G), 1,3;1,4 β-glucotriose A (G4G3G) and 1,3;1,4 β-glucotriose B (G3G4G), as well as cellotetraose (G4G4G4G) and laminaritetraose (G3G3G3G). Negative (CTRL -) and positive (GLCx100) control reactions were conducted with no substrate and with 250 mM GLC, respectively, and are reported on the left of the dashed line. Absorbance decrease at 520 nm was converted into percentage of reduced DCIP, using as 100% the absorbance of a solution containing no DCIP. Error bars represent the standard deviation of three independent experiments