Fig. 2

Sequence profiles of different alginate lyase families. Sequence profiles of A PL5 (structure template: 4F13) and B PL7 (structure template: 2CWS) were obtained by using, respectively, characterised enzymes. The sequence profile of C PL6 (structure template: 6A4O) was obtained using all PL6 enzymes in the CAZy database. In each sequence profile, the ordinate indicates the relative degree of conservation, while the abscissa shows the PDB ID of the template structure, as well as the type and sequence number of each amino acid. Each type of amino acid is represented by abbreviated letters with a corresponding colour (KRH, blue; DE, red; NQ, orange; WFY, green; others, black), where the same colour indicates similar physicochemical properties. The locations of ligand atoms interacting with the amino acid residues at each subsite are marked at the bottom, where CS stands for cleavage site. For each protein structure, residues within 5 Å around the active site are displayed and identified as the composition of active site architecture