Fig. 5

Ethanol production in S. cerevisiae using an increased cellulose load in the eSSF or mcSSF processes. Ethanol titers and percent theoretical yield obtained from S. cerevisiae in eSSF or mcSSF processes using a 2% cellulose content at both a 30 °C and b 42 °C with an enzyme load of 53 FPU/g cellulose. At this enzyme load, the eSSF process reaches at least 86% of the theoretical yield within 24 h at both temperatures. The eSSF and mcSSF processes are also compared at a lower enzyme load of 26 FPU/g cellulose at 30 °C (c), with the eSSF process exceeding the mcSSF titers by a factor of two after 36 h. Data is shown as mean values and error bars represent the standard deviation of three independent replicates