Table 2 List of genes and primers for RT-qPCR used in this study
From: Oxidative stress tolerance contributes to heterologous protein production in Pichia pastoris
Name | Primer sequence (5ʹ-3ʹ) |
|---|---|
ACT1 | F: TATTGAAGTTGAAGCCCTCTGAGC R: CCTTCCGTGTGCAAATGAAACAC |
CAT | F: TTCGACAACGCTAATCACGCTAAC R: TCACCTCAAACTCACCGAAAGCT |
SOD1 | F: TCGAACAATCCTCCGAAAGCAGC R: CCGTGGGTCTTACCAAATGGGT |
AHP1 | F: CTGGAGTCTTAGCTTGTGCTATTCC R: CGTCGATTTTCTCAATGAAAACAGG |
GLR1 | F: GGTGCAAAGACCCTTTTGATTGAAG R: GGTCCAGTTAAAGGAAAAATCGCC |
TRR1 | F: GGGTATGTTGGCTAACGGTATCG R: GGTCTCGGTAATGATCTCAGTGCC |
GSH2 | F: GCGATGTACCCCACCAATTTTGAG R: GCCCAGCCATTCCTGATTTTTGA |
ZWF1 | F: AGGGCGACGAGGACAAAGTTC R: GGGCAAAGCTAAGTAGAACAACCTG |
GND2 | F: GGTTTCACCGTCGTCGCTTACA R: CGGGATTACCAGCCTTGACCAATA |
Mxr1 | F: CCACAGTTGCAATACCAACAATCTC R: CGGAGAAGGAAGCTCTTCTTAGT |
Prm1 | F: ACAGGGACAATCTCTGAGTCTGA R: GTTGGAGCACTGTTGGGGTATAT |
Mit1 | F: GGTTCAAACTCTGGCAGTTCAGAT R: CCATTGGACATTCCATTGAAACCG |