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Table 2 Strains and plasmids used in this study

From: Function of ORFC of the polyketide synthase gene cluster on fatty acid accumulation in Schizochytrium limacinum SR21

Strains and plasmids Relevant genotype Source
E.coli strains
DH5α F-, φ80dlacZΔM15, Δ(lacZYA-argF) U169, deoR, recA1, endA1, hsdR17(rK-, mK +), phoA, supE44, λ-, thi-1, gyrA96, relA1 Takara
S. cerevisiae strains
YSG50 MATα, ade2-1, ade3Δ22, ura3-1, his3-11,15, trp1-1, leu2-3,112 and can1-100 A gift from Prof. Yuan
YSG50-C MATα, ade2-1, ade3Δ22, ura3-1, his3-11,15, trp1-1, leu2-3,112 and can1-101, orfC+ This study
Schizochytrium limacinum strains
SR21   ATCC
DH-overexpression strain ble+, DH+ This study
ER-overexpression strain ble+, ER+ This study
Plasmids
 pRS426 2μ, URA3, AmpR New England Biolabs
 pRS426-orfC 2μ, URA3, AmpR, ADH1p, CYC1t, orfC This study
 pBlueZeo-MAT AmpR, ZeoR, MAT+ Constructed by our lab [48]
 pBlueZeo-DH AmpR, ZeoR, DH+ This study
 pBlueZeo-ER AmpR, ZeoR, ER+ This study
  1. AmpR indicates ampicillin resistance, ZeoR indicates zeocin resistance