Table 2 Strains and plasmids used in this study

E.coli strains

DH5α

F-, φ80dlacZΔM15, Δ(lacZYA-argF) U169, deoR, recA1, endA1, hsdR17(rK-, mK +), phoA, supE44, λ-, thi-1, gyrA96, relA1

Takara

S. cerevisiae strains

YSG50

MATα, ade2-1, ade3Δ22, ura3-1, his3-11,15, trp1-1, leu2-3,112 and can1-100

A gift from Prof. Yuan

YSG50-C

MATα, ade2-1, ade3Δ22, ura3-1, his3-11,15, trp1-1, leu2-3,112 and can1-101, orfC⁺

This study

Schizochytrium limacinum strains

SR21

ATCC

DH-overexpression strain

ble⁺, DH⁺

This study

ER-overexpression strain

ble⁺, ER⁺

This study

Plasmids

 pRS426

2μ, URA3, Amp^R

New England Biolabs

 pRS426-orfC

2μ, URA3, Amp^R, ADH1p, CYC1t, orfC

This study

 pBlueZeo-MAT

Amp^R, Zeo^R, MAT⁺

Constructed by our lab [48]

 pBlueZeo-DH

Amp^R, Zeo^R, DH⁺

This study

 pBlueZeo-ER

Amp^R, Zeo^R, ER⁺

This study