Fig. 5

The phenotype of lignin reduction with biomass recovery is stable in the T2, T3 and T4 generations. a Cas9 copy number tested by qPCR for the transgenic T1 lines; the #57 and #83 lines are single copy insertion lines. b Selecting the single homozygous lines (underlined blue) in the T2 generation from #83 T1 seeds. c The total stem biomass of Col-0, ccr1–3, ccr1–3 ProSNBE:CCR1 and transgenic Crispr:CCR1:ProSNBE: ΔCCR1 T2, T3 and T4 lines. d The primary inflorescence stem height of the above lines. e The lignin content of the above lines. f The glucose release per seedling (based on stem inflorescence) of the above lines. In c–f, bars represent means and standard deviations, n = 6. Letters above bars indicate statistical significance by one-way ANOVA, p < 0.05