Fig. 5
From: Co-production of hydrogen and ethyl acetate in Escherichia coli
Fermentation profile of four strains engineered for ethyl acetate and hydrogen co-production in pH-controlled bioreactors with continuous gas stripping. Strains based on ΔldhA ΔackA (ΔΔ) with further modifications for improved hydrogen production, from left to right: inactivation of hycA, hyaAB and hybBC (ΔΔΔΔΔ), overexpression of fhlA (ΔΔ p3-fhlA) and a combination of knockouts and overexpression (ΔΔΔΔΔ p3-fhlA) producing trEat1 Wan N-13 were induced by 0.01 mM IPTG and cultivated under anaerobic conditions in minimal medium with 55 mM glucose as carbon source.. Experiments were performed as biological duplicates; error bars represent the standard deviation. Circles—compounds in liquid broth, triangle—compounds in off-gas