Fig. 6

Effect of modifications towards improved hydrogen production on product yields and productivities for main fermentation products, with from left to right: inactivation of hycA, hyaAB and hybBC, overexpression of fhlA and a combination of knockouts and overexpression. Strains producing trEat1 Wan N-13 were induced by 0.01 mM IPTG and grown under anaerobic conditions in minimal medium containing 55 mM glucose using pH-controlled bioreactors with 0.5-L working volume. a Product yields for ethyl acetate, hydrogen and formate in mol_product/mol_glucose after glucose depletion. The numbers above the bars represent the carbon recovery of the fermentations. b Volumetric productivities for ethyl acetate, hydrogen, and formate in mmol/L/h. Experiments were performed as biological duplicates or triplicates; error bars represent the standard deviation. trEat1 truncated Eat1 Wan N-13