Fig. 2

Purification and identification of the major NADH or NADPH-dependent ADH of Thermoanaerobacter sp. strain X514, AdhE and AdhB, respectively. a Denaturing SDS polyacrylamide gel electrophoresis of the fractions during isolation of AdhE (abundant from Thermoanaerobacter sp. strain X514. b The purified AdhE was separated by native PAGE under anaerobic conditions. c Fractions from the different purification steps of AdhB were separated by SDS-PAGE. 10 μg protein per lane; stained with Coomassie Brilliant Blue