Fig. 3From: Alcohol dehydrogenases AdhE and AdhB with broad substrate ranges are important enzymes for organic acid reduction in Thermoanaerobacter sp. strain X514Expression levels of the putative adh genes (with their corresponding gene number) and the aor gene (Teth514_1380) of Thermoanaerobacter sp. strain X514 during growth on 25Â mM glucose (black bars) and 25Â mM glucose plus 50Â mM isobutyrate (white bars). The transcript abundance of the 9 adh genes and the aor gene was analyzed with qRT-PCR, and is given relative to the abundance of the gene encoding for the DNA gyrase gyrA (Teth514_0010) as housekeeping gene (i.e., relative transcript level of gyrA equals 1). Teth514_0627, adhE; Teth514_0653, adhB; Teth514_0654, adhA. The average and standard deviation of three independent replicates are shownBack to article page