|
AdhE
|
AdhB
|
---|
Substrate
|
[U mg–1]
|
[U mg–1]
|
---|
|
NAD+
|
NAD+
|
NADP+
|
---|
Alcohol oxidation*
|
− CoA
|
+ CoA
| | |
Ethanol
|
2.2
|
3.3
|
0.1
|
8.8
|
2-Propanol
|
n. d
|
n. d
|
5.3
|
51.4
|
1-Propanol
|
2.0
|
4.8
|
n. d
|
n. d
|
1-Butanol
|
1.4
|
2.3
|
n. d
|
n. d
|
2-Butanol
|
0
|
n. d
|
n. d
|
n. d
|
2,3-Butanediol
|
0
|
n. d
|
n. d
|
n. d
|
Aldehyde/ketone reduction
|
NADH
|
NADPH
|
NADH
|
NADPH
|
Acetaldehyde
|
46.0
|
7.0
|
4.1
|
33.3
|
Propionaldehyde
|
45.3
|
n. d
|
0.9
|
29.9
|
Isobutyraldehyde
|
40.6
|
n. d
|
0.6
|
38.0
|
Butyraldehyde
|
29.7
|
n. d
|
0
|
24.4
|
Phenylacetaldehyde
|
n. d
|
n. d
|
0
|
2.3
|
Acetone
|
0
|
n. d
|
0.7
|
29.4
|
2,3-Butanedione
|
0
|
n. d
|
1.9
|
34.7
|
Aldehyde oxidation (+ CoA)
|
NAD+
|
NADP+
|
NAD+
|
NADP+
|
Acetaldehyde
|
10.5
|
1.1
|
0.1
|
0
|
Propionaldehyde
|
8.4
|
n. d
|
n. d
|
n. d
|
Isobutyraldehyde
|
7.3
|
n. d
|
n. d
|
n. d
|
Butyraldehyde
|
10.6
|
n. d
|
n. d
|
n. d
|
- Activities were determined in 50 mM Tris buffer (pH 7.5) supplemented with 2 mM DTE and 4 µM resazurin. Alcohol (200–500 mM) or aldehyde (10–50 mM) oxidation was determined with NAD(P)+ (2 mM) as electron acceptor, in the presence or absence of coenzyme A (0.2 mM; AdhE). Aldehyde or ketone (10 mM) reduction to alcohols was determined with NAD(P)H (0.5 mM) as electron donor; and absorption at 340 nm was measured. For details, please see “Methods” section. One unit (U) refers to one µmol of NADP+ reduced or NAD(P)H oxidized per minute. n. d.: not determined
- *Please note that CoA-dependent alcohol oxidation requires the reduction of 2 mol of NAD(P)+