Table 1 Oligonucleotides used in this study
From: The phenomenon of degeneration of industrial Trichoderma reesei strains
Name | Sequence (5′–3′) | Employment |
|---|---|---|
PCR1_fwd | TCCATCCCCATCCCGTTCTCCATCCATCCATG (32) | Sequencing of xyr1 locus |
PCR1_rev | CTAAACAAGATCGATCAGTACATG (24) | |
PCR2_fwd | ATTCAACGGGTACTGCTGGG (20) | |
PCR2_rev | GTTCAAGTCGTGCTCATCCAC (21) | |
PCR3_fwd | CCACCTGCCAACCAGGAGG (19) | |
PCR3_rev | TAGAGGGCCAGACCGGTTC (19) | |
epiactinTr_f | CTTCCCTCCTTTCCTCCCCCTCCAC | CHART-PCR |
epiactinTr_r | GCGACAGGTGCACGTACCCTCCATT | |
epicbh1_1Tr_f | AAGGGAAACCACCGATAGCAGTGTC | |
epicbh1_1Tr_r | TTTCACTTCACCGGAACAAACAAGC | |
epicbh1_2Tr_f | GGATCGAACACACTGCTGCCTTTAC | |
epicbh1_2Tr_r | GGTTTCTGTGCCTCAAAAGATGGTG | |
epicbh2_1Tr_f | CGGATCTAGGGCAGACTGGGCATTG | |
epicbh2_1Tr_r | GTGTAGTGTTGCGCTGCACCCTGAG | |
epicbh2_2Tr_f | TGCAGCGCAACACTACACGCAACAT | |
epicbh2_2Tr_r | TGCGCCTCATACAGGGTCACAGTCC | |
episar1Tr_f | GTCAGGAAATGCCGCACAAGCAAGA | |
episar1Tr_r | TGTGTTTTACCGCCTTGGCCTTTGG | |
epixyr1_1Tr_f | CCTTTGGCCATCTACACAAGAGCAA | |
epixyr1_1Tr_r | CGCAATTTTTATTGCTGTTCGCTTC | |
epixyr1_2Tr_f | CCGACAGCAGCAGTAGTCAGGTTTT | |
epixyr1_2Tr_r | TAGGCAGAATAGCGACGGAGAGGAT | |
epixyr1_3Tr_f | GGCAGCCGTGTAGCTTGTCA | |
epixyr1_3Tr_r | GGAATCAAACCGTCGCCTCTT | |
act_fw | TGAGAGCGGTGGTATCCACG | qPCR |
act_rev | GGTACCACCAGACATGACAATGTTG | |
cbh1f | GATGATGACTACGCCAACATGCTG | |
cbh1r | ACGGCACCGGGTGTGG | |
cbh2f | CTATGCCGGACAGTTTGTGGTG | |
cbh2r | GTCAGGCTCAATAACCAGGAGG | |
sar1fw | TGGATCGTCAACTGGTTCTACGA | |
sar1rev | GCATGTGTAGCAACGTGGTCTTT | |
xyr1f | CCCATTCGGCGGAGGATCAG | |
xyr1r | CGAATTCTATACAATGGGCACATGGG |