Skip to main content

Table 2 Kinetic parameters and specific activity of xylanases against sugarcane and corncob xylan at 40 °C

From: Improvement of XYL10C_∆N catalytic performance through loop engineering for lignocellulosic biomass utilization in feed and fuel industries

Substrate

Sugarcane xylan

Corncob xylan

Enzymes

Km (mg/mL)

Vmax (μmol/min·mg)

kcat/Km (mL/s·mg)

Specific activity (U/mg)

Km (mg/mL)

Vmax (μmol/min·mg)

kcat/Km (mL/s·mg)

Specific activity (U/mg)

XYL10C_∆N

1.52 ± 0.04

730 ± 38

320 ± 28

740 ± 33

1.89 ± 0.14

810 ± 52

300 ± 15

770 ± 63

M53S/F54L

1.63 ± 0.17

1920 ± 81

750 ± 41

1940 ± 77

1.44 ± 0.16

1880 ± 88

830 ± 48

1980 ± 91

N207G

1.91 ± 0.08

1210 ± 56

400 ± 33

1120 ± 82

1.55 ± 0.11

1290 ± 72

530 ± 47

1370 ± 62

M53S/F54L/N207G

1.18 ± 0.10

2030 ± 79

1100 ± 81

2080 ± 85

1.03 ± 0.04

2090 ± 84

1290 ± 81

2080 ± 92

  1. The kinetic values are shown as means ± standard deviations (n = 3)
Back to article page

Biotechnology for Biofuels and Bioproducts

ISSN: 2731-3654

Contact us