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Fig. 3 | Biotechnology for Biofuels

Fig. 3

From: CRISPR/Cas9-mediated genome editing directed by a 5S rRNA–tRNAGly hybrid promoter in the thermophilic filamentous fungus Humicola insolens

Fig. 3

Verification of xyr1 gene disruption in the Y1/Δku70 strain. A Schematic diagram of xyr1 disruption verification. hyg: hygromycin B resistance gene. B PCR verification of xyr1 disruption using the primer pair xyr1YS/xyr1YA. M: DNA molecular mass marker; lane 1: negative control; lane 2: H. insolens Y1; lane 3: Y1/Δku70; lanes 4–7: four representative xyr1 disrupted mutants

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Biotechnology for Biofuels and Bioproducts

ISSN: 2731-3654

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