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Table 1 Cell growth and xylose consumption of strains expressing different xylose isomerase (XI) variants

From: Directed evolution and secretory expression of xylose isomerase for improved utilisation of xylose in Saccharomyces cerevisiae

Strain

Mutations

Growth (OD600)

Consumed xylose (g)

Optimal pH

WT

–

15.3 ± 0.5

9.0 ± 0.3

7.0

XI-76

E56A, I252M

18.6 ± 0.7

13.4 ± 0.5

5.0

XI-E56A

E56A

16.5 ± 0.5

10.8 ± 0.4

6.0

XI-I252M

I252M

16.3 ± 0.4

10.6 ± 0.4

7.0

  1. Data shown represent the mean from triplicate experiments ± SD
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Biotechnology for Biofuels and Bioproducts

ISSN: 2731-3654

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