Fig. 2

Patterns of fluorescence emission in strains transformed with pMF_002 vector and analyzed by flow cytometric. A Schematic representation of the pMF_002 vector and simplification of expected results. B Fluorescence pattern of the diploid strain JAY270 without pMF_002 vector. C Fluorescence pattern of the diploid strain JAY270 containing the vector pMF_002. D Fluorescence pattern of the haploid strain JAY289 (MATa) transformed with vector pMF_002. E Fluorescence pattern of the haploid JAY290 (MATα) transformed with pMF_002. F Fermentation pattern of a heterogeneous population comprising both haploids JAY289 (MATa) and JAY290 (MATα) transformed with vector pMF_002. Specific MATa cells emitting green fluorescence are expected to form a population on quadrant 1 (Q1) while specific MATα cells emitting orange fluorescence are expected to fall on quadrant 3 (Q3)