Fig. 2

Cell growth of recombinant strains of 8b-PiXI-xylB and 8b-RsXI-xylB in RMX5. Strains were cultured in 300 μL RMX5 with 50 μg/mL spectinomycin in 500 μL microporous honeycomb panel by Bioscreen C instrument at 30 °C. The induced concentration of tetracycline is 0, 2, and 6 μg/mL, and the plate was shaken before measuring the OD_{600 nm} every 3 min. Three replicates were performed for each experiment. The diagram in the panel represented plasmid constructs expressing both XI and XK genes controlled by the Ptet promoter (pink triangle) and rrnB T1 terminator (grey triangle). PiXI and RsXI represented the xylose isomerase (XI) genes from Piromyces sp. E2, and protists in the Reticulitermes speratus hindgut, respectively; xylB was xylulokinase gene from E. coli. Plasmid containing the reporter gene EGFP was used as the control