Fig. 4

Accumulated metabolites for co-cultures of C. churrovis paired with M. bryantii versus monocultures of C. churrovis upon harvest. HPLC data are shown for co-culture and monoculture grown on each substrate. No formate was observed in co-culture on any substrate, suggesting that M. bryantii is capable of metabolizing formate. Trace amounts of ethanol were present in the cultures but fell below the 0.1 g/L limit of detection. This, in conjunction with increased levels of acetate in co-culture, indicates that some of the PFLs upregulated in co-cultures grown on xylan and fructose may be functioning within the hydrogenosome