Fig. 7

Overview of putative carbon metabolism involving the induced mitochondria-mediated C4-like CCM in LC-cultivated P. tricornutum. This proposed metabolic map was based on enzymic activity, transcriptional abundances, predicted subcellular location and proteomic analysis. The upregulated enzymes are indicated by red color, and the downregulated enzymes are indicated by green color, while the non-detected enzymes are indicated by grey color. Red arrow and green arrow indicate the increase or decrease activity (or transcriptional abundances) of the enzyme under LC conditions, respectively. Predicted subcellular localization of P. tricornutum genes related to the biophysical CCM, C4-like-CCM, central carbon metabolism, photorespiration, the ornithine–urea cycle and PYR metabolism are listed in an additional excel sheet Additional file 2: Table S2. SLC4-2: solute carrier protein; CA: carbonic anhydrase; ME: malic enzyme; MDH: malate dehydrogenase; PEPCK: phosphoenolpyruvate carboxylase kinase; PEPC: phosphoenolpyruvate carboxylase; PPDK: pyruvate orthophosphate dikinase; AAT: aspartate aminotransferase; PK: pyruvate kinase; PYC: pyruvate carboxylase; PDH: pyruvate dehydrogenase; PDC: pyruvate decarboxylase; CS: citrate synthase; GAPDH: glyceraldehyde 3-phosphate dehydrogenase; PGK: phosphoglycerate kinase; PGAM: phosphoglycerate mutase; GK: glycerate kinase; PGP: 2-phosphoglycolate phosphatases; GOX: glycolate oxidase; HPR: hydroxypyruvate reductase/glycerate dehydrogenase; GDC: glycine decarboxylase; SHMT: serine hydroxymethyltransferase; SPAT: serine-pyruvate aminotransferase; AGAT: alanine- glyoxylate aminotransferase; CPS: carbamoyl-phosphate synthase; OTC: ornithine transcarbamoylase; ASS: argininosuccinate synthase; ASL: argininosuccinatelyase; ARG: arginase; ACS: acetyl-CoA synthase; ALDH: aldehyde dehydrogenase; GLNA: glutamine synthase; GLN: glutamate synthase; G3P: glyceraldehyde-3-phosphate; 1,3-PGA: 1,3-biphosphoglyceride; 3-PGA: 3-phosphoglycerate; 2-PGA: 2-phosphoglycerate; PEP: phosphoenolpyruvate; PYR: pyruvate; OAA: oxaloacetate; MAL: malate; RuBP: ribulose-1,5-biphosphate; CarbP: carbamoyl phosphate; PP pathway: pentose phosphate pathway; TCA cycle: tricarboxylic acid cycle