Fig. 3

Identification of protein acetylation in S. cerevisiae under different culture modes. A Culture mode design and the cell sample acquisition flow chart. D9 indicates that the yYST12 strain cells was cultured in synthetic complete-glucose medium (SG) for 9 days (d) in continuous passages; D9X6 indicates that the yYST12 strain cells was cultured in SG medium for 9 days in continuous passages and then the cells was transferred to synthetic complete-xylose medium (SX) for 6 days in continuous passages; D9X6D9 indicates that the yYST12 strain cells was cultured in SG medium for 9 days, then the cells was transferred to SX medium for 6 days, and again the cells was transferred to SG medium for 9 days. B Comparative plot of lysine acetylation site changes in the yYST12 strain under different culture modes. C Least-squares method employed for analyzing the protein acetylation and variable importance index (VIP) plot according to the importance of the acetylation site