Table 1 Ethanol production by engineered cyanobacteria

Synechocystis strain

PCC 6803

pdc_Zm + adh_Syn (slr1192)

glgC + phaC + phaE

0.986

Dual copies; 2.96 g/L in 3 d; highest OD₇₃₀ ~ 50; pnblA promoter

[27]

 ~ 0.2

Dual copies; 5.5 g/L in 26 d; highest OD₇₃₀ ~ 4; pnblA promoter; Nitrogen starvation

[27]

phaA + phaB

0.285

Dual copies; 2.6 g/L in 9 d; ppsbAII promoter

[28, 29]

0.255

Optimised pdc:adh expression ratio to 2:1; 2.3 g/L in 9 d; prbc promoter

[30]

0.212

Dual copies; 5.5 g/L in 26 d; highest OD₇₃₀ ~ 12; prbc promoter

[24]

Plasmid

0.06

Overexpressed FBA and TK; 1.2 g/L in 20 d; pnrsB promoter for pdc and adh

[31]

0.236

Patented; 7.1 g/L in 30 d; ziaA promoter; construction #1318

[32]

0.235

Patented; 4.7 g/L in 20 d; corT promoter

[32]

0.261

4.7 g/L in 18 d; highest OD₇₃₀ ~ 13; pVZ325 plasmid; petJ promoter

[33]

0.097

Patented; 3.6 g/L in 37 d; pVZ321b plasmid and others; petJ promoter

[34]

pdc_Zm + adhII_Zm

pdc/adhII

0.0766

0.46 g/L in 6 d; psbA2 promoter

[35]

pdc_Sc + adh_Syn (slr0942)

glgC + phaA

0.157

Co-culture of knockout & ethanologenic strains; 4.7 g/L in 30 d; psbA promoter

[36]

0.137

4.1 g/L in 30 d; psbA promoter

[36]

Synechococcus strain

PCC 7002

pdc_Zm + adh_Syn (slr1192)

glgA1 + glgA2

0.220

Dual copies; 2.2 g/L in 10 d; highest OD₇₃₀ ~ 4; prbc promoter

[37]

-

-

0.410

Patented, JCC1581_B isolate; 5.62 g/L in 13.7 d;

[38]

PCC 7942¹

pdc_Zm + adh_Zm

Genomic

0.076

25% CO₂ sparging; 0.23 g/L in 3 d; prbcL promoter

[39]

Plasmid

0.008

0.23 g/L in 28 d; prbsLS promoter

[15]

ABICyano1²

-

Plasmid

0.552

Patented, plasmid TK504; copper inducible promoter

[40]