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Table 1 Ethanol production by engineered cyanobacteria

From: Combinatorial use of environmental stresses and genetic engineering to increase ethanol titres in cyanobacteria

Cyanobacterium Ethanologenic cassette Genotype Ethanol rate
(g/L/d) 		Comments Refs.
Synechocystis strain  
PCC 6803 pdcZm + adhSyn (slr1192) glgC + phaC + phaE 0.986 Dual copies; 2.96 g/L in 3 d; highest OD730 ~ 50; pnblA promoter [27]
     ~ 0.2 Dual copies; 5.5 g/L in 26 d; highest OD730 ~ 4; pnblA promoter; Nitrogen starvation [27]
   phaA + phaB 0.285 Dual copies; 2.6 g/L in 9 d; ppsbAII promoter [28, 29]
    0.255 Optimised pdc:adh expression ratio to 2:1; 2.3 g/L in 9 d; prbc promoter [30]
    0.212 Dual copies; 5.5 g/L in 26 d; highest OD730 ~ 12; prbc promoter [24]
   Plasmid 0.06 Overexpressed FBA and TK; 1.2 g/L in 20 d; pnrsB promoter for pdc and adh [31]
    0.236 Patented; 7.1 g/L in 30 d; ziaA promoter; construction #1318 [32]
    0.235 Patented; 4.7 g/L in 20 d; corT promoter [32]
    0.261 4.7 g/L in 18 d; highest OD730 ~ 13; pVZ325 plasmid; petJ promoter [33]
    0.097 Patented; 3.6 g/L in 37 d; pVZ321b plasmid and others; petJ promoter [34]
  pdcZm + adhIIZm pdc/adhII 0.0766 0.46 g/L in 6 d; psbA2 promoter [35]
  pdcSc + adhSyn (slr0942) glgC + phaA 0.157 Co-culture of knockout & ethanologenic strains; 4.7 g/L in 30 d; psbA promoter [36]
    0.137 4.1 g/L in 30 d; psbA promoter [36]
Synechococcus strain
PCC 7002 pdcZm + adhSyn (slr1192) glgA1 + glgA2 0.220 Dual copies; 2.2 g/L in 10 d; highest OD730 ~ 4; prbc promoter [37]
  - - 0.410 Patented, JCC1581_B isolate; 5.62 g/L in 13.7 d; [38]
PCC 79421 pdcZm + adhZm Genomic 0.076 25% CO2 sparging; 0.23 g/L in 3 d; prbcL promoter [39]
   Plasmid 0.008 0.23 g/L in 28 d; prbsLS promoter [15]
ABICyano12 - Plasmid 0.552 Patented, plasmid TK504; copper inducible promoter [40]
  1. 1Synechococcus elongatus strain; 2Phylogeny of the novel cyanobacterial isolate ABICyano1 is closest to Cyanobacterium aponinum PCC 10,605 and Cyanobacterium aponinum ETS-03 [16]
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Biotechnology for Biofuels and Bioproducts

ISSN: 2731-3654

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