Engineering Rhodosporidium toruloides for limonene production

Table 1 Limonene titers in different hosts

Host	Limonene synthase	Stereo isomer	Pathway	Direct precursor of LS	Carbon source	Cultivation mode	Titer (mg/L)	Productivity (mg/L/h)^a	Yield (mg/g)	Reference
Escherichia coli BL21 (DE3)	Mentha spicata	S	MEP&MVA	GPP	Glycerol	Bioreactor Fed-batch	3630	151	< 10^b	Rolf et al. [9]
Saccharomyces cerevisiae EGY48	Citrus limon	R	MVA	GPP	Glucose	Bioreactor Fed batch	2580	3.36^b	NA	Dusséaux et al. [11]
Yarrowia lipolytica Po1f	Agastache rugosa	R	MVA	NPP	Glycerol &Citrate	Bioreactor; Fed-batch	165.3	1.15^a	41.3^b	Cheng et al. [8]
Synechococcus elongatus UTEX 2973 (mutated)	Mentha spicata	S	MEP	GPP	CO2;	250 μmol photons/m²/s	16.4	0.34	NA	Lin et al. [14]
Rhodosporidium toruloides IFO0880	Abies grandis	S	MVA	GPP	Glucose	Shake flask batch	Trace	NA	NA	Zhuang et al. [20]
Rhodosporidium toruloides np11	Citrus limon	R	MVA	NPP	Glucose	5-mL test-tube; batch	393.5	0.66	19.675	This work

ISSN: 2731-3654