Fig. 3From: Cocktail biosynthesis of triacylglycerol by rational modulation of diacylglycerol acyltransferases in industrial oleaginous AurantiochytriumTLC analysis of lipids from H1246 and its transformants. A H1246 and its transformants were cultivated without fatty acid-fed. B H1246 and its transformants were cultivated under DHA-fed condition. Line 1, H1246 harboring the empty plasmid pYES2; line 2, H1246 expressing yeast DGA1 (DGAT2) gene; line 3, line 4, line 5, and line 6, mutant strain H1246 expressing DGAT2A, DGAT2B, DGAT2C, and DGAT2D gene, respectively. C TLC analysis of substrate preference of DGAT2C by feeding with C18:2, C20:4, C20:5, and C22:6Back to article page