Skip to main content

Table 1 Strains and plasmids used in this study

From: Cocktail biosynthesis of triacylglycerol by rational modulation of diacylglycerol acyltransferases in industrial oleaginous Aurantiochytrium

Strain or plasmid Description Reference or source
Strains   
 Escherichia coli DH5α Strain used for plasmid construction Invitrogen
Aurantiochytrium sp.
 SD116
Wild type [13]
 SD116::DGAT2A Overexpression of DGAT2A gene in SD116 This study
 SD116::DGAT2B Overexpression of DGAT2B gene in SD116 This study
 SD116::DGAT2C Overexpression of DGAT2C gene in SD116 This study
 SD116::DGAT2D Overexpression of DGAT2D gene in SD116 This study
Saccharomyces cerevisiae
 H1246 The TAG-deficient quadruple of S. cerevisiae. Genotype: MATα are1– Δ::HIS3 are2-Δ:: LEU2 dga1 -Δ:: Kan MX4 lro1-Δ::TRP1 ADE2 [34]
 pYES2 H1246 harboring the empty plasmid pYES2 [34]
 ScDGA1 H1246 expressing yeast DGA1 gene [34]
 DGAT2A H1246 expressing DGAT2A gene This study
 DGAT2B H1246 expressing DGAT2B gene This study
 DGAT2C H1246 expressing DGAT2C gene This study
 DGAT2D H1246 expressing DGAT2D gene This study
Plasmids
 pGZC-1 Plasmid carrying the zeoR gene expression cassette and 18 s rDNA homologous arm [35]
 pDGAT2A Derived from pDGAT2C, containing DGAT2A expression cassette This study
 pDGAT2B Derived from pDGAT2A, containing DGAT2B expression cassette This study
 pDGAT2C Derived from pGZC-1, containing DGAT2C expression cassette This study
 pDGAT2D Derived from pDGAT2C, containing DGAT2D expression cassette This study