Table 1 Strains and plasmids used in this study
Strain or plasmid | Description | Reference or source |
|---|---|---|
Strains | ||
Escherichia coli DH5α | Strain used for plasmid construction | Invitrogen |
Aurantiochytrium sp. SD116 | Wild type | [13] |
SD116::DGAT2A | Overexpression of DGAT2A gene in SD116 | This study |
SD116::DGAT2B | Overexpression of DGAT2B gene in SD116 | This study |
SD116::DGAT2C | Overexpression of DGAT2C gene in SD116 | This study |
SD116::DGAT2D | Overexpression of DGAT2D gene in SD116 | This study |
Saccharomyces cerevisiae | ||
H1246 | The TAG-deficient quadruple of S. cerevisiae. Genotype: MATα are1– Δ::HIS3 are2-Δ:: LEU2 dga1 -Δ:: Kan MX4 lro1-Δ::TRP1 ADE2 | [34] |
pYES2 | H1246 harboring the empty plasmid pYES2 | [34] |
ScDGA1 | H1246 expressing yeast DGA1 gene | [34] |
DGAT2A | H1246 expressing DGAT2A gene | This study |
DGAT2B | H1246 expressing DGAT2B gene | This study |
DGAT2C | H1246 expressing DGAT2C gene | This study |
DGAT2D | H1246 expressing DGAT2D gene | This study |
Plasmids | ||
pGZC-1 | Plasmid carrying the zeoR gene expression cassette and 18 s rDNA homologous arm | [35] |
pDGAT2A | Derived from pDGAT2C, containing DGAT2A expression cassette | This study |
pDGAT2B | Derived from pDGAT2A, containing DGAT2B expression cassette | This study |
pDGAT2C | Derived from pGZC-1, containing DGAT2C expression cassette | This study |
pDGAT2D | Derived from pDGAT2C, containing DGAT2D expression cassette | This study |