Fig. 4

Effect of tungsten (W) addition on cell growth and ectoine production in M. alcaliphilum 20ZDP. Time course of the biomass (dry cell weight; DCW), ectoine, and hydroxyectoine production from methane by M. alcaliphilum 20ZDP cultivated in W-free medium (a) and in W-added medium (b), respectively. The following symbols were used: DCW (●), ectoine (▲), and hydroxyectoine (X). All experiments were performed in triplicate and the range of the raw data was within ± 5% of the average