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Fig. 4 | Biotechnology for Biofuels and Bioproducts

Fig. 4

From: Boosting the acetol production in methanotrophic biocatalyst Methylomonas sp. DH-1 by the coupling activity of heteroexpressed novel protein PmoD with endogenous particulate methane monooxygenase

Fig. 4

Bioconversion of acetone to acetol under batch conditions. A Growth rate of Methylomonas sp. DH-1 wild-type (DH-1_WT) and recombinant (DH-1_IT6 and DH-1_ΔP450) under the cultivation conditions of 30% (v/v) methane with the addition of acetone to the titer of 10 g/L after the first 24-h cultivation. B Titer of acetone and acetol of DH-1_WT, DH-1_IT6, and DH-1_ΔP450 under cultivation of 30% (v/v) methane with the addition of acetone to the titer of 10 g/L after 24 h. C Accumulation of intracellular pyruvate extracted from cell mass of DH-1_WT, DH-1_IT6, and DH-1_ΔP450 at 120 and 168 h timepoints. Error bars represent the standard deviation. Three independent biological experiments were performed in triplicate and one representative experiment was chosen for figure production. *P < 0.05, significant differences were noted with two-way ANOVA statistical analysis

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